17/05/2019
The Human Resources Strategy for Researchers

Candida albicans “on-chip”: Biomechanical approach of morphogenetic plasticity of the yeast C. albic

This job offer has expired


  • ORGANISATION/COMPANY
    CNRS
  • RESEARCH FIELD
    Engineering
    Physics
    Technology
  • RESEARCHER PROFILE
    First Stage Researcher (R1)
  • APPLICATION DEADLINE
    07/06/2019 23:59 - Europe/Brussels
  • LOCATION
    France › PARIS 05
  • TYPE OF CONTRACT
    Temporary
  • JOB STATUS
    Full-time
  • HOURS PER WEEK
    35
  • OFFER STARTING DATE
    01/10/2019

We are looking for a highly motivated student who is eager to work at the interface between physics and biology. Among others, the candidate will learn microbiology techniques and microfabrication. The thesis will be in between two laboratories: the LAAS-CNRS (Toulouse), under the supervision of M. Delarue, and the Institut Curie – IPGG (Paris) under the supervision of C. Villard. The first half of the PhD program will be at LAAS-CNRS, where the student would be studying the yeast-to-hyphal transition under well-defined physico-chemical conditions, learning microfabrication in the state-of-the-art 1500m2 clean room. The second half of the PhD will be at Institut Curie/Institut Pierre-Gilles de Gennes for microfluidics, investigating the role of confinement in hyphal growth and the pseudo-hyphal-to-hyphal transition. The PhD student would sign in the PSL-“Physique Ile de France” doctoral school in Paris, and regular bimonthly meetings will be organized, either in Paris or in Toulouse.

CONTEXT
Candida albicans is an opportunistic pathogen yeast that exists in three morphotypes: rugby- shaped yeasts, elongated filamentous hyphae, and intermediate pseudo-hyphae types (see Graphical abstract). The switch from one morphotype to another one has been associated with benign (candidiasis) to deed-seated infections in which C. albicans cells invade biological tissues.
Different regions of the body have different physico-chemical characteristics, resulting notably in various sources of confinement and mechanical stresses that C. albicans cells may experience. Recently, we have shown that the switch from one morphotype to another one was highly dependent on mechanics, raising the question of the role of such stresses in promoting or restraining virulence.
PROJECT
The goal of this PhD project is to investigate morphotype regulation under mechanical stress. To this end, various microfluidic devices will be developed in order to control the physico-chemical environment cells are subjected to. In particular, the yeast-to-hyphal and the pseudo-hyphal-to- hyphal transitions will be investigated. The results from these biophysical studies will serve as a basis to study C. albicans invasiveness into mammalian cells within “organ-on-a-chip”-like devices. This PhD will take place into a larger collaborative network involving Pasteur Institute in Paris (C. d'Enfert team) and the theoretical group in Physico-Chimie Curie (P. Sens).

Required Research Experiences

  • RESEARCH FIELD
    Engineering
  • YEARS OF RESEARCH EXPERIENCE
    None
  • RESEARCH FIELD
    Physics
  • YEARS OF RESEARCH EXPERIENCE
    None
  • RESEARCH FIELD
    Technology
  • YEARS OF RESEARCH EXPERIENCE
    None

Offer Requirements

  • REQUIRED EDUCATION LEVEL
    Engineering: Master Degree or equivalent
    Physics: Master Degree or equivalent
    Technology: Master Degree or equivalent
  • REQUIRED LANGUAGES
    FRENCH: Basic
Work location(s)
1 position(s) available at
Laboratoire Physico-Chimie Curie
France
PARIS 05

EURAXESS offer ID: 409276
Posting organisation offer ID: 9648

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